Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS.

@article{Chen2014SitespecificCO,
  title={Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS.},
  author={Rui Chen and Deeptee Seebun and Mingliang Ye and Hanfa Zou and Daniel Figeys},
  journal={Journal of proteomics},
  year={2014},
  volume={103},
  pages={194-203}
}
UNLABELLED Glycosylation of membrane proteins plays an important role in cellular behaviors such as cell-cell interaction, immunologic recognition and cell signaling. However, the effective extraction of membrane proteins, the selective isolation of glycopeptides and the mass spectrometric characterization of glycosylation are challenging with current analytical techniques. In this study, a systematic approach was developed which combined: an integrated hydrophilic interaction chromatography… CONTINUE READING