Site-specific biotinylation of purified proteins using BirA.


The binding between biotin and streptavidin or avidin is one of the strongest known non-covalent biological interactions. The (strept)avidin-biotin interaction has been widely used for decades in biological research and biotechnology. Therefore labeling of purified proteins by biotin is a powerful way to achieve protein capture, immobilization, and… (More)
DOI: 10.1007/978-1-4939-2272-7_12


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@article{Fairhead2015SitespecificBO, title={Site-specific biotinylation of purified proteins using BirA.}, author={Michael Fairhead and Mark Howarth}, journal={Methods in molecular biology}, year={2015}, volume={1266}, pages={171-84} }