Site-specific bacterial chromosome engineering: ΦC31 integrase mediated cassette exchange (IMCE).

@article{Heil2012SitespecificBC,
  title={Site-specific bacterial chromosome engineering: ΦC31 integrase mediated cassette exchange (IMCE).},
  author={John R Heil and Jiujun Cheng and Trevor C. Charles},
  journal={Journal of visualized experiments : JoVE},
  year={2012},
  volume={61}
}
The bacterial chromosome may be used to stably maintain foreign DNA in the mega-base range. Integration into the chromosome circumvents issues such as plasmid replication, plasmid stability, plasmid incompatibility, and plasmid copy number variance. This method uses the site-specific integrase from the Streptomyces phage (Φ) C31. The ΦC31 integrase catalyzes a direct recombination between two specific DNA sites: attB and attP (34 and 39 bp, respectively). This recombination is stable and does… CONTINUE READING