Site-specific analysis of heteronuclear Overhauser effects in microcrystalline proteins.

Abstract

Relaxation parameters such as longitudinal relaxation are susceptible to artifacts such as spin diffusion, and can be affected by paramagnetic impurities as e.g. oxygen, which make a quantitative interpretation difficult. We present here the site-specific measurement of [(1)H](13)C and [(1)H](15)N heteronuclear rates in an immobilized protein. For methyls… (More)
DOI: 10.1007/s10858-014-9843-1

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