Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics.

@article{SchulteZweckel2014SitespecificRA,
  title={Site-specific, reversible and fluorescent immobilization of proteins on CrAsH-modified surfaces for microarray analytics.},
  author={Janine Schulte-Zweckel and Federica Rosi and Domalapally Sreenu and Hendrik V Schr{\"o}der and Christof M. Niemeyer and Gemma Triola},
  journal={Chemical communications},
  year={2014},
  volume={50 84},
  pages={
          12761-4
        }
}
A novel technique for protein immobilization onto CrAsH-modified surfaces is presented. This approach enables an efficient, reversible and fluorogenic immobilization of proteins. Moreover, expressed proteins can also be directly immobilized from cellular lysates without prior purification. The immobilized proteins are suitable for protein-protein interaction studies and the fluorescence enhancement upon immobilization can be employed for the direct detection of the immobilized protein without… 

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