Site-selective scission of human genome by artificial restriction DNA cutter.

@article{Ito2009SiteselectiveSO,
  title={Site-selective scission of human genome by artificial restriction DNA cutter.},
  author={Kenichiro Ito and Hitoshi Katada and Narumi Shigi and Makoto Komiyama},
  journal={Chemical communications},
  year={2009},
  volume={43},
  pages={
          6542-4
        }
}
By using an artificial restriction DNA cutter which is composed of Ce(iv)/EDTA and two pseudo-complementary peptide nucleic acid strands (pcPNAs), only one target site in the whole genome of human beings (one site in the X chromosome) was selectively hydrolyzed. 
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28 Citations
Background Citations
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Methods Citations
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28 Citations

Design and Applications of Artificial Restriction DNA Cutters for Site-Selective Scission of Genomes

By combining Ce(IV)/EDTA complex and pseudo-complementary peptide nucleic acid (pcPNA), an artificial restriction DNA cutter which hydrolyzes targeted phosphodiester linkages in double-stranded DNA...

Site-selective scission of human genome using PNA-based artificial restriction DNA cutter.

This chapter describes the method of site-selective scission of human genomic DNA using ARCUT, a completely chemistry-based artificial restriction DNA cutter by combining a pair of pseudo-complementary PNA strands and Ce(IV)/EDTA complex (molecular scissors).

Artificial site-selective DNA cutters to manipulate single-stranded DNA

With the cutters composed of two oligonucleotide additives and Ce(IV)/EDTA, long single-stranded DNA can be selectively cut at target site and manipulated according to the needs.

Site-specific Manipulation of Mitochondrial DNA by Artificial Restriction DNA Cutter

Site-specific gene manipulation using homologous recombination induced by a double-strand break in DNA is expected to be applied to mitochondrial genome manipulation. In this study, by using a chem...

Clipping of predetermined fragments from the human genome by S1 nuclease-PNA combinations.

By combining S1 nuclease with two strands of pseudo-complementary peptide nucleic acid (pcPNA), the whole human genome was selectively cut at targeted sites, and desired fragments were clipped from…

Site-selective Scission of Double-stranded DNA by Combining a Triplex-forming bis-PNA and Ce(IV)/EDTA

By combining triplex-forming bis-peptide nucleic acid (bis-PNA) with Ce(IV)/EDTA, double-stranded DNA was selectively hydrolyzed at a predetermined homopyrimidine/homopurine site, which is difficul...

Artificial Restriction DNA Cutters ( ARCUT ) as New Tools to Manipulate Human Genome

Artificial restriction DNA cutter (ARCUT) which cuts double-stranded DNA at one desired site using Ce(IV)/EDTA complex as molecular scissors and a pair of pseudo-complementary PNAs, and their scission-site is determined by simple Watson-Crick rule.

Artificial DNA cutters for DNA manipulation and genome engineering.

This tutorial review provides recent developments in artificial cutters for site-selective scission of DNA with the focus on chemistry-based DNA cutters, since their site- selectivity is much higher than that of naturally occurring restriction enzymes and also their scission site is freely chosen.

Site-selective DNA hydrolysis induced by a metal-free peptide nucleic acid-cyclen conjugate.

A metal-free artificial restriction DNA cutter which is composed of cyclen and classical peptide nucleic acid (PNA) was synthesized. Analysis of DNA cleavage products indicates the site-selective…

Chemical modifications of artificial restriction DNA cutter (ARCUT) to promote its in vivo and in vitro applications

  • M. Komiyama
  • Biology, Chemistry
    Artificial DNA, PNA & XNA
  • 2014
Strong potential ofpcPNA for further applications in vivo and in vitro has been confirmed and new strategies using conventional PNA, in place of pcPNA, as site-selective activator are presented.

References

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Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity

The artificial restriction DNA cutter (ARCUT) method to cut double-stranded DNA at designated sites has been developed and the location of the scission site and the site-specificity are almost freely tunable, and there is no limitation to the size of DNA substrate.

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