Site-selected mutagenesis of the Drosophila second chromosome via plasmid rescue of lethal P-element insertions.

@article{Guo1996SiteselectedMO,
  title={Site-selected mutagenesis of the Drosophila second chromosome via plasmid rescue of lethal P-element insertions.},
  author={Yi-qing Guo and Andrea Gillan and Tibor T{\"o}r{\"o}k and Istv{\'a}n M{\'a}rton Kiss and Julian A. T. Dow and Kim Kaiser},
  journal={Genome research},
  year={1996},
  volume={6 10},
  pages={972-9}
}
This paper describes a fast and efficient approach to correlating cloned genes with mutant phenotypes in Drosophila. We make use of a large collection D. melanogaster lines with recessive lethal insertions of a P[lacW] transposon on their second chromosome. Within this collection there clearly must be many insertions corresponding to Drosophila genes that have been cloned and characterized, e.g., via homology with cloned mammalian genes, but for which mutant phenotypes have yet to be identified… CONTINUE READING

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