Site-directed tryptophan fluorescence reveals the solution structure of tear lipocalin: evidence for features that confer promiscuity in ligand binding.

@article{Gasymov2001SitedirectedTF,
  title={Site-directed tryptophan fluorescence reveals the solution structure of tear lipocalin: evidence for features that confer promiscuity in ligand binding.},
  author={Oktay K. Gasymov and Adil R. Abduragimov and Taleh N. Yusifov and Ben J. Glasgow},
  journal={Biochemistry},
  year={2001},
  volume={40 49},
  pages={14754-62}
}
The solution structure of human TL was deduced from the position of the emission peaks after site-directed tryptophan fluorescence (SDTF). The fluorescent amino acid tryptophan was sequentially substituted for each native amino acid in the sequence. Characteristic periodicities for eight beta-strands that comprise the beta-barrel and three alpha-helices… CONTINUE READING