Site-directed spin-labeling of transmembrane domain VII and the 4B1 antibody epitope in the lactose permease of Escherichia coli.

@article{Vo1997SitedirectedSO,
  title={Site-directed spin-labeling of transmembrane domain VII and the 4B1 antibody epitope in the lactose permease of Escherichia coli.},
  author={J{\"u}rgen Vo\ss and Wayne L. Hubbell and Jordi Hern{\'a}ndez-Borrell and H Ronald Kaback},
  journal={Biochemistry},
  year={1997},
  volume={36 49},
  pages={15055-61}
}
Functional lactose permease mutants containing single Cys residues at positions 233-255 and a biotin acceptor domain at the C terminus were solubilized in dodecyl beta-d-maltopyranoside and purified by avidin affinity chromatography. Each mutant protein was derivatized with a thiol-selective nitroxide reagent and examined by conventional and power saturation electron paramagnetic resonance spectroscopy (EPR). The EPR spectral line shapes and the influence of nonpolar O2 or polar potassium… CONTINUE READING