Site-directed spin labeling of membrane proteins.

  title={Site-directed spin labeling of membrane proteins.},
  author={Enrica Bordignon},
  journal={Topics in current chemistry},
EPR spectroscopy of site-directed spin labeled membrane proteins is at present a common and valuable biophysical tool to study structural details and conformational transitions under conditions relevant to function. EPR is considered a complementary approach to X-ray crystallography and NMR because it provides detailed information on (1) side chain dynamics with an exquisite sensitivity for flexible regions, (2) polarity and water accessibility profiles across the membrane bilayer, and (3… CONTINUE READING