Site-directed recombination via bifunctional PNA-DNA conjugates.

  title={Site-directed recombination via bifunctional PNA-DNA conjugates.},
  author={Faye A. Rogers and Karen M. Vasquez and Michael Egholm and Peter M. Glazer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={99 26},
Site-specific DNA binding molecules offer the potential for genetic manipulation of mammalian cells. Peptide nucleic acids (PNAs) are a DNA mimic in which the purine and pyrimidine bases are attached to a polyamide backbone. PNAs bind with high affinity to single-stranded DNA via Watson-Crick base pairing and can form triple helices via Hoogsteen binding to DNAPNA duplexes. Dimeric bis-PNAs capable of both strand invasion and triplex formation can form clamp structures on target DNAs. As a… CONTINUE READING


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Anticancer Drug Des

  • P. E. Nielsen, M. Egholm, R. H. Berg, O. Buchardt
  • 1993

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