Site-directed mutagenesis of the putative human muscarinic M2 receptor binding site.

@article{Heitz1999SitedirectedMO,
  title={Site-directed mutagenesis of the putative human muscarinic M2 receptor binding site.},
  author={Freddy Heitz and James Anthony Holzwarth and J P Gies and Rebecca M. Pruss and Susanne Trumpp-Kallmeyer and Marcel Hibert and Chantal Guenet},
  journal={European journal of pharmacology},
  year={1999},
  volume={380 2-3},
  pages={183-95}
}
Experimental probing of the model of the muscarinic M2 receptor binding site proposed by Hibert et al. [Hibert, M.F., Trumpp-Kallmeyer, S., Bruinsvels, A., Hoflak, K., 1991. Three-dimensional models of neurotransmitter G-binding protein-coupled receptors. Mol. Pharmacol. 40, 8-15.] was achieved by mutating each amino-acid proposed to interact with muscarinic ligands. Pharmacological analysis of the different mutant receptors transiently expressed in human embryonic kidney (HEK/293) cells was… CONTINUE READING