Site-directed mutagenesis of conserved residues of Clostridium thermocellum endoglucanase CelC.

@article{Navas1992SitedirectedMO,
  title={Site-directed mutagenesis of conserved residues of Clostridium thermocellum endoglucanase CelC.},
  author={Jon A. Navas and Pierre B{\'e}guin},
  journal={Biochemical and biophysical research communications},
  year={1992},
  volume={189 2},
  pages={
          807-12
        }
}
Four conserved residues of Clostridium thermocellum endoglucanase CelC were replaced by site-directed mutagenesis. Proteins mutated in His-90, Asn-139 and Glu-140 showed strongly reduced activity, in agreement with predictions of sequence alignments. Mutations in Glu-140 did not result in any detectable change in Km, or apparent size, suggesting that Glu-140 is directly involved in catalysis. The pH optimum of the proteins carrying the Glu-140/Ala and Glu140/Gln mutations was lower than that of… CONTINUE READING
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