Site-directed mutagenesis of benzalacetone synthase. The role of the Phe215 in plant type III polyketide synthases.

@article{Abe2003SitedirectedMO,
  title={Site-directed mutagenesis of benzalacetone synthase. The role of the Phe215 in plant type III polyketide synthases.},
  author={Ikuro Abe and Yukie Sano and Yusuke Takahashi and Hiroshi Noguchi},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 27},
  pages={25218-26}
}
Benzalacetone synthase (BAS) and chalcone synthase (CHS) are plant-specific type III polyketide synthases (PKSs) that share approximately 70% amino acid sequence identity. BAS catalyzes a one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce a diketide benzalacetone, whereas CHS performs sequential condensations with three malonyl-CoA to generate a tetraketide chalcone. A homology model suggested that BAS has the same overall fold as CHS with cavity volume almost… CONTINUE READING
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