Site-directed mutagenesis of active site contact residues in a hydrolytic abzyme: evidence for an essential histidine involved in transition state stabilization.

@article{Miyashita1997SitedirectedMO,
  title={Site-directed mutagenesis of active site contact residues in a hydrolytic abzyme: evidence for an essential histidine involved in transition state stabilization.},
  author={Hiroshi Miyashita and Takane Hara and Ryuji Tanimura and Shiro Fukuyama and Christine Cagnon and Atsuko Kohara and Ikuo Fujii},
  journal={Journal of molecular biology},
  year={1997},
  volume={267 5},
  pages={1247-57}
}
Specific molecular interactions involved in catalysis by antibody 6D9 were investigated by site-directed mutagenesis. The catalytic antibody 6D9, which was generated against a transition state analog (III), hydrolyzes a non-bioactive chloramphenicol monoester derivative (I) to produce chloramphenicol (II). Construction of a three-dimensional molecular model of 6D9 and sequence comparison within a panel of related antibodies suggested candidates for catalytic residues, His (L27d), Tyr (L32), Tyr… CONTINUE READING

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