Site-directed mutagenesis of BmK AGP-SYPU1: the role of two conserved Tyr (Tyr5 and Tyr42) in analgesic activity.

@article{Deng2014SitedirectedMO,
  title={Site-directed mutagenesis of BmK AGP-SYPU1: the role of two conserved Tyr (Tyr5 and Tyr42) in analgesic activity.},
  author={Li Deng and Hong-xia Zhang and Y Wang and Rong Zhang and Xue Wen and Yong-Bo Song and Yong-Shan Zhao and Lin Ma and Chun-Fu Wu and Jing-hai Zhang},
  journal={The protein journal},
  year={2014},
  volume={33 2},
  pages={157-64}
}
In this study, the role of two conversed tyrosines (Tyr5 and Tyr42) from the scorpion toxin BmK AGP-SYPU1 was investigated with an effective Escherichia coli expression system. Site-directed mutagenesis was used to individually substitute Tyr5 and Tyr42 with hydrophobic or hydrophilic amino acids, and the extent to which these scorpion toxin BmK AGP-SYPU1 tyrosines contribute to analgesic activity was evaluated. The results of the mouse-twisting test showed that Tyr5 and Tyr42 are associated… CONTINUE READING

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The role of Ser54 in the antinociceptive activity of BmK9, a neurotoxin from the scorpion Buthus martensii Karsch

  • YQ Wang, Z Hao, +7 authors J Zhang
  • Toxicon
  • 2011
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