Site-directed mutagenesis confirms the involvement of carboxylate groups in the disassembly of tobacco mosaic virus.

@article{Culver1995SitedirectedMC,
  title={Site-directed mutagenesis confirms the involvement of carboxylate groups in the disassembly of tobacco mosaic virus.},
  author={James N. Culver and William O. Dawson and K Plonk and Gerald Stubbs},
  journal={Virology},
  year={1995},
  volume={206 1},
  pages={724-30}
}
Electrostatic repulsion between carboxylate groups across subunit interfaces has for many years been recognized as important in the disassembly of simple plant viruses. In the coat protein of tobacco mosaic virus (TMV), the amino acids Glu50 and Asp77 have been proposed as examples of such carboxylate groups. Site-directed mutagenesis has been used to replace these amino acids by Gln and Asn, respectively. Increased virion stability, together with reduced infectivity and reduced capacity for… CONTINUE READING

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