Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells

@article{Thomas1987SitedirectedMB,
  title={Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells},
  author={Kirk R. Thomas and Mario R. Capecchi},
  journal={Cell},
  year={1987},
  volume={51},
  pages={503-512}
}
We mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the neomycin resistance (neor) gene was introduced into an exon of a cloned fragment of the Hprt gene and used to transfect ES cells. Among the G418r colonies, 1/1000 were also resistant to the base analog 6-thioguanine (6-TG). The G418r, 6-TGr cells were all shown to be Hprt- as the result of homologous recombination with the… Expand
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