Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli.

@article{Li2008SitedirectedMB,
  title={Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli.},
  author={Jing Li and Chunhua Li and Wei Xiao and Dongxia Yuan and Gang Wan and Lixin Ma},
  journal={Analytical biochemistry},
  year={2008},
  volume={373 2},
  pages={389-91}
}
A rapid site-directed mutagenesis strategy using homologous recombination and DpnI digestion of the template in Escherichia coli is described. Briefly, inverse polymerase chain reaction amplification of the entire circular plasmid was performed by mutagenic primers with overlapping sequences ( approximately 15 bp) for generating PCR products with approximately 15 bp of homology on the terminal ends. On direct transformation of the amplified PCR products into restriction endonuclease DpnI… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

Similar Papers

Loading similar papers…