• Corpus ID: 37541457

Site-directed mutagenesis and expression in Escherichia coli of WMAI-1, a wheat monomeric inhibitor of insect alpha-amylase.

  title={Site-directed mutagenesis and expression in Escherichia coli of WMAI-1, a wheat monomeric inhibitor of insect alpha-amylase.},
  author={Federico Garcia-Maroto and Pilar Carbonero and Francisco Garc{\'i}a-Olmedo},
  journal={Plant molecular biology},
  volume={17 5},
The wheat monomeric inhibitor WMAI-1 (syn. 0.28) produced in Escherichia coli using the pT7-7 expression vector has the correct N-terminal sequence and the same electrophoretic mobility and specific activity towards the alpha-amylase from the insect Tenebrio molitor as the native WMAI-1 isolated from wheat. This confirms that the native inhibitor is not glycosylated and contradicts claims that a putative glycosyl moiety was essential for inhibition. Thirteen mutants have been obtained at six… 
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