Site-directed genome modification: derivatives of DNA-modifying enzymes as targeting tools.

@article{Coates2005SitedirectedGM,
  title={Site-directed genome modification: derivatives of DNA-modifying enzymes as targeting tools.},
  author={Craig J. Coates and Joseph M L Kaminski and James Bradley Summers and David J Segal and A Dusty Miller and Andreas F. Kolb},
  journal={Trends in biotechnology},
  year={2005},
  volume={23 8},
  pages={407-19}
}
The modification of mammalian genomes is an important goal in gene therapy and animal transgenesis. To generate stable genetic and biochemical changes, the therapeutic genes or transgenes need to be incorporated into the host genome. Ideally, the integration of the foreign gene should occur at sites that ensure their continual expression in the absence of… CONTINUE READING