Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.

@article{Chiu2004SitedirectedLM,
  title={Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.},
  author={Joyce Chiu and P. E. March and Ryan S. Lee and Daniel Tillett},
  journal={Nucleic acids research},
  year={2004},
  volume={32 21},
  pages={e174}
}
Site-directed, Ligase-Independent Mutagenesis (SLIM) is a novel PCR-mediated mutagenesis approach that can accommodate all three sequence modification types (insertion, deletion and substitution). The method utilizes an inverse PCR amplification of the template by two tailed long primers and two short primers in a single reaction with all steps carried out in one tube. The tailed primers are designed to contain the desired mutation on complementary overhangs at the terminus of PCR products… CONTINUE READING

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In Vitro Mutagenesis Protocols, 2nd edn

  • J. Braman
  • 2002

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