Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity


The unique reactivity of two sortase enzymes, SrtA(staph) from Staphylococcus aureus and SrtA(strep) from Streptococcus pyogenes, is exploited for site-specific labeling of a single polypeptide with different labels at its N and C termini. SrtA(strep) is used to label the protein's C terminus at an LPXTG site with a fluorescently labeled dialanine… (More)
DOI: 10.1021/ja902681k