Site-Specific Hydrogen Exchange in a Membrane Environment Analyzed by Infrared Spectroscopy.

  title={Site-Specific Hydrogen Exchange in a Membrane Environment Analyzed by Infrared Spectroscopy.},
  author={Esther S. Brielle and Isaiah Tuvia Arkin},
  journal={The journal of physical chemistry letters},
  volume={9 14},
Hydrogen exchange is a powerful method to examine macromolecules. In membrane proteins, exchange can distinguish between solvent-accessible and -inaccessible residues due to shielding by the hydrophobic environment of the lipid bilayer. Herein, rather than examining which residues undergo hydrogen exchange, we employ a protocol that enables the full deuteration of all polar hydrogens in a membrane protein. We then measure the impact of hydrogen exchange on the shift of the amide I vibrational… 

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