Site-Selective Conjugation of Native Proteins with DNA.

  title={Site-Selective Conjugation of Native Proteins with DNA.},
  author={Julie B Trads and Thomas T\orring and Kurt Vesterager Gothelf},
  journal={Accounts of chemical research},
  volume={50 6},
Conjugation of DNA to proteins is increasingly used in academia and industry to provide proteins with tags for identification or handles for hybridization to other DNA strands. Assay technologies such as immuno-PCR and proximity ligation and the imaging technology DNA-PAINT require DNA-protein conjugates. In DNA nanotechnology, the DNA handle is exploited to precisely position proteins by self-assembly. For these applications, site-selective conjugation is almost always desired because fully… CONTINUE READING