Site-Directed Dichroism As a Method for Obtaining Rotational and Orientational Constraints for Oriented Polymers

@inproceedings{Arkin1997SiteDirectedDA,
  title={Site-Directed Dichroism As a Method for Obtaining Rotational and Orientational Constraints for Oriented Polymers},
  author={Isaiah T. Arkin and Kevin Mackenzie and Axel T. Bru and nger},
  year={1997}
}
We present the theory of site-directed dichroism and its application to the determination of rotational and orientational constraints for oriented polypeptides such as transmembrane helices. Infrared spectroscopy dichroism measurements of single amide I vibrational modes corresponding to 13C-labeled sites within the polypeptide contain information about the helix tilt and rotation angles. This information is readily extracted by analysis of the dichroism of a set of sites along the peptide… CONTINUE READING
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