Single vs. swarm detection of microparticles and exosomes by flow cytometry

  title={Single vs. swarm detection of microparticles and exosomes by flow cytometry},
  author={Edwin van der Pol and M. J. C. Van GEMERT and Auguste Sturk and R. Nieuwland and T. G. Van LEEUWEN},
  journal={Journal of Thrombosis and Haemostasis},
See also Harrison P, Gardiner C. Invisible vesicles swarm within the iceberg. This issue, pp 916‐8. 

Flow cytometric analysis of extracellular vesicle subsets in plasma: impact of swarm by particles of non‐interest

Fluorescence‐based flow cytometric analysis is suitable to detect low abundant EV subsets and interference of particles of non‐interest can be monitored by analyzing serial dilutions.

A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles

Plasma contains cell‐derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential applications as disease biomarker. However, the enumeration of EVs

Standardization of extracellular vesicle measurements by flow cytometry through vesicle diameter approximation

A model is applied to convert ambiguous scatter units to EV diameter in nanometer and outperforms polystyrene beads for comparability of platelet EV concentrations.

MIFlowCyt‐EV: The Next Chapter in the Reporting and Reliability of Single Extracellular Vesicle Flow Cytometry Experiments

A working group composed of members from the international societies of extracellular vesicles, advancement of cytometry, and thrombosis and haemostasis developed the recent MIFlowCyt-EV framework position statement, with the aim to improve reporting and reliability of single EV flow cytometry experiments.

Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS

Optimized protocols for EV labeling that increase the signal‐to‐noise ratio of EVs by removing residual antibody are presented and Protocols for alignment of high‐resolution jet‐in‐air flow cytometers are provided.

Rate zonal centrifugation can partially separate platelets from platelet‐derived vesicles

Centrifugation is commonly used as a first step to enrich biomarkers from blood and affects the yield and purity of biomarkers, for example, isolation of platelets results in co‐isolation with extracellular vesicles (EVs).

Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration

Plasma and other body fluids contain membranous extracellular vesicles (EVs), which are considered to derive from activated or apoptotic cells. EVs participate in physiological and pathological

Innovation in detection of microparticles and exosomes

This review focuses on the relatively high throughput detection of vesicles in suspension by flow cytometry, resistive pulse sensing, and nanoparticle tracking analysis, and four methods that are not commercially available: Raman microspectroscopy, micro nuclear magnetic resonance, small‐angle X‐ray scattering (SAXS), and anomalous SAXS.

Intriguing Biomedical Applications of Synthetic and Natural Cell-Derived Vesicles: A Comparative Overview

The significant role of a vesicle is well recognized; however, only lately has the advancement in biomedical applications started to uncover their usefulness. Although the concept of vesicles origi...



Atomic force microscopy: a novel approach to the detection of nanosized blood microparticles

See also Freyssinet J‐M, Toti F. Membrane microparticle determination: at least seeing what’s being sized! This issue, pp 311–4.

Platelet microparticles are heterogeneous and highly dependent on the activation mechanism: Studies using a new digital flow cytometer

Platelet‐derived microparticles (MPs) are believed to play an important role in coagulation and inflammatory disorders. Unfortunately, MP size renders them difficult to study and analyze by

Single particle high resolution spectral analysis flow cytometry

There are several types of analytical measurements that would benefit from a more comprehensive and flexible approach to spectral analysis including, but certainly not limited to spectral deconvolution of overlapping emission spectra, fluorescence resonance energy transfer measurements, metachromic dye analysis, free versus bound dye resolution, and Raman spectroscopy.

Optical and non‐optical methods for detection and characterization of microparticles and exosomes

Several (combinations of) methods can detect clinically relevant properties of microparticles and exosomes and should be further explored and validated by comparing measurement results so that accurate, reliable and fast solutions come within reach.

Overcoming limitations of microparticle measurement by flow cytometry.

This review addresses flow cytometry limitations in microparticle measurement that may be subdivided into three domains: sizing, probing, and counting and covers the various standardization strategies and technological improvements that have been proposed to overcome these limitations.

More on: calibration for the measurement of microparticles: value of calibrated polystyrene beads for flow cytometry‐based sizing of biological microparticles

Calibration for the measurement of microparticles: needs, interests, and limitations of calibrated polystyrene beads for flow cytometry‐based quantification of biologicalmicroparticles.

Flow cytometer for measurement of the light scattering of viral and other submicroscopic particles

  • H. Steen
  • Biology
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2004
This work has shown that light scattering measurement of viruses is generally not feasible with current flow cytometers due to their small size, and the problem is aggravated by the fact that the light scattering of particles in this size range falls off with roughly the sixth power of their linear dimensions.

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis

More on: calibration for the measurement of microparticles: needs, interests, and limitations of calibrated polystyrene beads for flow cytometry‐based quantification of biological microparticles

Calculating the value of calibrated polystyrene beads for flow cytometry‐based sizing of biological microparticles and the authors respond are responded to.