Single-tube nested quantitative PCR: a rational and sensitive technique for detection of retroviral DNA. Application to RERV-H/HRV-5 and confirmation of its rabbit origin.

@article{Forsman2003SingletubeNQ,
  title={Single-tube nested quantitative PCR: a rational and sensitive technique for detection of retroviral DNA. Application to RERV-H/HRV-5 and confirmation of its rabbit origin.},
  author={Anna Forsman and Dmitrijs Uzameckis and Lars R{\"o}nnblom and Eva Baecklund and Anna {\AA}leskog and Amarinder Bindra and Ruediger Pipkorn and Sandra Lejniece and Svetlana V Kozireva and Modra Murovska and Jonas Blomberg},
  journal={Journal of virological methods},
  year={2003},
  volume={111 1},
  pages={1-11}
}
It was reported earlier that a few patients suffering from non-Hodgkin's lymphoma had low amounts of DNA from the so-called fifth human exogenous retrovirus, HRV-5. A sensitive and rational method for large-scale screening for HRV-5 DNA was therefore developed. It is a single-tube nested quantitative PCR (stnQPCR), which uses two functionally isolated primer pairs and one probe target distinct from related endogenous retroviral sequences, yet encompassing known HRV-5 variation, allowing optimal… CONTINUE READING