Single-tube nested PCR in the diagnosis of tuberculosis.

  title={Single-tube nested PCR in the diagnosis of tuberculosis.},
  author={C M Chan and K. Y. Yuen and Kian Sing Chan and Wing Cheong Yam and Kook Hyun Yim and Wan Fai Ng and Margaret H L Ng},
  journal={Journal of clinical pathology},
  volume={49 4},
AIMS To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment sensitivity and specificity without increasing the risk of amplicon contamination, was evaluated. Specimens were initially tested for… CONTINUE READING
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