Single-stranded shuttle phagemid for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA induces targeted G.C-->T.A transversions in simian kidney cells.

@article{Moriya1993SinglestrandedSP,
  title={Single-stranded shuttle phagemid for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA induces targeted G.C-->T.A transversions in simian kidney cells.},
  author={Masaaki Moriya},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 3},
  pages={1122-6}
}
  • Masaaki Moriya
  • Published 1993 in
    Proceedings of the National Academy of Sciences…
A single-stranded shuttle vector has been developed for the purpose of investigating translesional events in mammalian cells. The vector is designed to permit site-specific introduction of defined DNA lesions between a gene for neomycin resistance and its promoter. Efficiencies of translesional synthesis in simian kidney cells (COS) and Escherichia coli are established by determining the number of neomycin- and ampicillin-resistant colonies recovered, respectively, after introduction of a… CONTINUE READING