Single-stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics.

@article{Zheng2013SinglestrandedDA,
  title={Single-stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics.},
  author={Tianqing Zheng and Hao Jiang and Peng Wu},
  journal={Bioconjugate chemistry},
  year={2013},
  volume={24 6},
  pages={859-64}
}
In this communication, we report a new class of cleavable linker based on automatically synthesized, single-stranded DNAs. We incorporated a DNA oligo into an azide-functionalized biotin (biotin-DNA-N3) and used the probe to enrich for alkyne-tagged glycoproteins from mammalian cell lysates. Highly efficient and selective release of the captured proteins from streptavidin agarose resins was achieved using DNase treatment under very mild conditions. A total of 36 sialylated glycoproteins were… CONTINUE READING