Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.

@article{LealKlevezas1995SinglestepPF,
  title={Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.},
  author={Diana Sara Leal-Klevezas and Irma Olivia Mart{\'i}nez-V{\'a}zquez and Ahid{\'e} L{\'o}pez-Merino and Juan Pablo Ricardo Mart{\'i}nez-Soriano},
  journal={Journal of clinical microbiology},
  year={1995},
  volume={33 12},
  pages={3087-90}
}
A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in… CONTINUE READING

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