Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers.

@article{Knowles2010SingleSG,
  title={Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers.},
  author={Michelle K. Knowles and Sebastian Barg and Li Wan and Mitsuharu Midorikawa and Xiaoyong Chen and Wolfhard Almers},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2010},
  volume={107 48},
  pages={
          20810-5
        }
}
Before secretory vesicles undergo exocytosis, they must recruit the proteins syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in the plasma membrane. GFP-labeled versions of both proteins cluster at sites where secretory granules have docked. Single-particle tracking shows that minority populations of both molecules are strongly hindered in their mobility, consistent with their confinement in nanodomains. We measured the fluorescence of granule-associated clusters, the fluorescence… CONTINUE READING

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