Single protein production in living cells facilitated by an mRNA interferase.

@article{Suzuki2005SinglePP,
  title={Single protein production in living cells facilitated by an mRNA interferase.},
  author={Motoo Suzuki and Junjie Zhang and Mohan Liu and Nancy A. Woychik and M. Inouye},
  journal={Molecular cell},
  year={2005},
  volume={18 2},
  pages={
          253-61
        }
}
We designed a single-protein production (SPP) system in living E. coli cells that exploits the unique properties of MazF, a bacterial toxin that is an ssRNA- and ACA-specific endoribonuclease. In effect, MazF functions as an "mRNA interferase," because it efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a precipitous drop in total protein synthesis. Concomitant expression of MazF and a target gene engineered to encode an ACA-less mRNA results in sustained and high… CONTINUE READING
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