Single pass sequencing and physical and genetic mapping of human brain cDNAs

@article{Khan1992SinglePS,
  title={Single pass sequencing and physical and genetic mapping of human brain cDNAs},
  author={Akbar S. Khan and Andrea S. Wilcox and Mihael H. Polymeropoulos and Janet Hopkins and Tamara J. Stevens and Misi Robinson and Arto K. Orpana and James M. Sikela},
  journal={Nature Genetics},
  year={1992},
  volume={2},
  pages={180-185}
}
We have performed single pass sequencing of 1,024 human brain cDNAs, over 900 of which seem to represent new human genes. Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene–specific STS primers derived from 3′ untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene–associated polymorphic markers… CONTINUE READING

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