Single particle electron microscopy in combination with mass spectrometry to investigate novel complexes of membrane proteins.

@article{Arteni2005SinglePE,
  title={Single particle electron microscopy in combination with mass spectrometry to investigate novel complexes of membrane proteins.},
  author={A. A. Arteni and Marc Nowaczyk and Julia E-M Lax and Roman Kouřil and Matthias R{\"o}gner and Egbert J. Heel Boekema},
  journal={Journal of structural biology},
  year={2005},
  volume={149 3},
  pages={325-31}
}
Large data sets of molecular projections of the membrane proteins Photosystem I and Photosystem II from cyanobacteria were analyzed by single particle electron microscopy (EM). Analysis resulted in the averaging of 2D projections from the purified complexes but also in the simultaneous detection and averaging of 2D projections from large contaminating complexes, which were present in frequencies as low as 0.1%. Among them T-shaped and L-shaped contaminants were found. The L-shaped particles… CONTINUE READING