Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Förster distance dependence and subpopulations.

@article{Deniz1999SinglepairFR,
  title={Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of F{\"o}rster distance dependence and subpopulations.},
  author={Ashok A Deniz and Maxime Dahan and Jocelyn R Grunwell and Taekjip Ha and Ann Elise Faulhaber and Daniel S Chemla and Shimon Weiss and Peter G. Schultz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 7},
  pages={3670-5}
}
Photon bursts from single diffusing donor-acceptor labeled macromolecules were used to measure intramolecular distances and identify subpopulations of freely diffusing macromolecules in a heterogeneous ensemble. By using DNA as a rigid spacer, a series of constructs with varying intramolecular donor-acceptor spacings were used to measure the mean and distribution width of fluorescence resonance energy transfer (FRET) efficiencies as a function of distance. The mean single-pair FRET efficiencies… CONTINUE READING

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