Single molecule unzipping of coiled coils: sequence resolved stability profiles.

@article{Bornschlgl2006SingleMU,
  title={Single molecule unzipping of coiled coils: sequence resolved stability profiles.},
  author={Thomas Bornschl{\"o}gl and Matthias Rief},
  journal={Physical review letters},
  year={2006},
  volume={96 11},
  pages={118102}
}
We use a high resolution atomic force microscopy technique to mechanically unzip and rezip single coiled-coil proteins. This allows us to read off the complete stability profile of the protein turn by turn. We investigated three coiled coils with different length as well as a point mutation and find force fluctuations between 9 and 15 pN that can be directly related to the amino-acid sequences. An equilibrium model previously applied to DNA fully describes the mechanical unzipping process… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Sequence-resolved free energy profiles of stress-bearing vimentin intermediate filaments.

Proceedings of the National Academy of Sciences of the United States of America • 2014
View 1 Excerpt

From mechanical folding trajectories to intrinsic energy landscapes of biopolymers.

Proceedings of the National Academy of Sciences of the United States of America • 2013
View 12 Excerpts

Similar Papers

Loading similar papers…