Single-molecule tracking of sub-millisecond domain motion in calmodulin.

  title={Single-molecule tracking of sub-millisecond domain motion in calmodulin.},
  author={Brian D. Slaughter and Ramona J Bieber-Urbauer and Carey K Johnson},
  journal={The journal of physical chemistry. B},
  volume={109 26},
We used single-pair fluorescence resonance energy transfer (spFRET) to track distance changes between domains of fluorescently labeled calmodulin (CaM) on the sub-millisecond time scale. In most cases, CaM remained in the same conformational substate over time periods of up to 1 ms, showing that conformational interchange occurs on a longer time scale. However, in some instances, apparent transitions between conformational substates could be detected. The magnitude of sub-millisecond motion… CONTINUE READING

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