Single-molecule tracking of mRNA exiting from RNA polymerase II.

Abstract

Single-pair fluorescence resonance energy transfer was used to track RNA exiting from RNA polymerase II (Pol II) in elongation complexes. Measuring the distance between the RNA 5' end and three known locations within the elongation complex allows us determine its position by means of triangulation. RNA leaves the polymerase active center cleft via the previously proposed exit tunnel and then disengages from the enzyme surface. When the RNA reaches lengths of 26 and 29 nt, its 5' end associates with Pol II at the base of the dock domain. Because the initiation factor TFIIB binds to the dock domain and exit tunnel, exiting RNA may prevent TFIIB reassociation during elongation. RNA further extends toward the linker connecting to the polymerase C-terminal repeat domain (CTD), which binds the 5'-capping enzyme and other RNA processing factors.

5 Figures and Tables

01002002008200920102011201220132014201520162017
Citations per Year

834 Citations

Semantic Scholar estimates that this publication has 834 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Andrecka2008SinglemoleculeTO, title={Single-molecule tracking of mRNA exiting from RNA polymerase II.}, author={Joanna Andrecka and Robert Y. Lewis and Florian Brueckner and Elisabeth Lehmann and Patrick Cramer and Jens Michaelis}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2008}, volume={105 1}, pages={135-40} }