Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes

@inproceedings{Beliveau2015SinglemoleculeSI,
  title={Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes},
  author={Brian J Beliveau and Alistair N. Boettiger and Maier S. Avenda{\~n}o and Ralf Jungmann and Ruth B. McCole and Eric F Joyce and Caroline Kim-Kiselak and Fr{\'e}d{\'e}ric Bantignies and Chamith Y. Fonseka and Jelena Erceg and Mohammed A. Hannan and Hien G. Hoang and David Colognori and Jeannie T Lee and William M. Shih and Peng Yin and Xiaowei Zhuang and Chao-Ting Wu},
  booktitle={Nature communications},
  year={2015}
}
Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization of single-copy regions of the genome using two single-molecule super-resolution methodologies. We then introduce a robust and reliable… CONTINUE READING