Single-molecule studies reveal the function of a third polymerase in the replisome

@article{Georgescu2011SinglemoleculeSR,
  title={Single-molecule studies reveal the function of a third polymerase in the replisome},
  author={Roxana E Georgescu and Isabel Kurth and Mike E O'Donnell},
  journal={Nature Structural &Molecular Biology},
  year={2011},
  volume={19},
  pages={113-116}
}
The Escherichia coli replisome contains three polymerases, one more than necessary to duplicate the two parental strands. Using single-molecule studies, we reveal two advantages conferred by the third polymerase. First, dipolymerase replisomes are inefficient at synthesizing lagging strands, leaving single-strand gaps, whereas tripolymerase replisomes fill strands almost to completion. Second, tripolymerase replisomes are much more processive than dipolymerase replisomes. These features account… CONTINUE READING
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