Single-molecule studies of repressor-DNA interactions show long-range interactions.

  title={Single-molecule studies of repressor-DNA interactions show long-range interactions.},
  author={Yayu Wang and Jonas O. Tegenfeldt and Walter Reisner and Robert Riehn and X Guan and Ling Guo and Ido Golding and Edward C. Cox and James C. Sturm and Robert H Austin},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={102 28},
We have performed single-molecule studies of GFP-LacI repressor proteins bound to bacteriophage lambda DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to be 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the… CONTINUE READING

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