• DOI:10.1038/nmeth.3475
Corpus ID: 205423980

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells

@article{Koenig2015SinglemoleculeSO,
  title={Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells},
  author={I. Koenig and A. Zarrine-Afsar and Mikayel Aznauryan and Andrea Soranno and Bengt Wunderlich and Fabian Dingfelder and Jakob C St{\"u}ber and A. Pl{\"u}ckthun and D. Nettels and B. Schuler},
  journal={Nature Methods},
  year={2015},
  volume={12},
  pages={773-779}
}

I. Koenig, A. Zarrine-Afsar, +7 authors B. Schuler

Published 2015

Medicine

Nature Methods

Single-molecule methods have become widely used for quantifying the conformational heterogeneity and structural dynamics of biomolecules in vitro. Their application in vivo, however, has remained challenging owing to shortcomings in the design and reproducible delivery of labeled molecules, the range of applicable analysis methods, and suboptimal cell culture conditions. By addressing these limitations in an integrated approach, we demonstrate the feasibility of probing protein dynamics from… CONTINUE READING

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