Single molecule spectroscopic characterization of GFP-MUT2 mutant for two-photon microscopy applications.

@article{Cannone2004SingleMS,
  title={Single molecule spectroscopic characterization of GFP-MUT2 mutant for two-photon microscopy applications.},
  author={Fabio Cannone and Michele Caccia and Sara Bologna and Alberto Diaspro and Giuseppe Chirico},
  journal={Microscopy research and technique},
  year={2004},
  volume={65 4-5},
  pages={186-93}
}
Green Fluorescent Protein (GFP) mutants are extensively used in optical microscopy studies of in vivo biological processes in cells. Nonetheless, blinking and bleaching of the GFP chromophore at the single molecule level greatly limits its usefulness. We have worked out what we think are the best experimental conditions for the use of the GFP mutant, GFP-mut2, as a single molecule marker in two-photon excitation measurements. We have measured molecular brightness, excited state lifetime… CONTINUE READING