Single-molecule sequencing detection of N6-methyladenine in microbial reference materials

@inproceedings{McIntyre2019SinglemoleculeSD,
  title={Single-molecule sequencing detection of N6-methyladenine in microbial reference materials},
  author={Alexa B. R. McIntyre and N Vasiliev Alexander and Kirill Grigorev and Daniela Bezdan and Heike Sichtig and Charles Y. Chiu and Christopher E. Mason},
  booktitle={Nature Communications},
  year={2019}
}
  • Alexa B. R. McIntyre, N Vasiliev Alexander, +4 authors Christopher E. Mason
  • Published in Nature Communications 2019
  • Biology, Medicine
  • The DNA base modification N6-methyladenine (m6A) is involved in many pathways related to the survival of bacteria and their interactions with hosts. Nanopore sequencing offers a new, portable method to detect base modifications. Here, we show that a neural network can improve m6A detection at trained sequence contexts compared to previously published methods using deviations between measured and expected current values as each adenine travels through a pore. The model, implemented as the… CONTINUE READING

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