Single-molecule sequencing and chromatin conformation capture enable de novo reference assembly of the domestic goat genome

@article{Bickhart2017SinglemoleculeSA,
  title={Single-molecule sequencing and chromatin conformation capture enable de novo reference assembly of the domestic goat genome},
  author={Derek M. Bickhart and Benjamin D. Rosen and Sergey Koren and Brian L. Sayre and Alex R Hastie and Saki Chan and Joyce Lee and Ernest T. Lam and Ivan Liachko and Shawn T. Sullivan and Joshua N. Burton and Heather J Huson and John C Nystrom and Christy M. Kelley and Jana L. Hutchison and Yang Zhou and Jiajie Sun and Alessandra Cris{\`a} and F Abel Ponce de Le{\'o}n and John C Schwartz and John A. Hammond and Geoffrey C. Waldbieser and Steven G. Schroeder and George E. Liu and Maitreya J. Dunham and Jay Shendure and Tad S. Sonstegard and Adam M. Phillippy and Curtis P Van Tassell and Timothy L Smith},
  journal={Nature Genetics},
  year={2017},
  volume={49},
  pages={643-650}
}
The decrease in sequencing cost and increased sophistication of assembly algorithms for short-read platforms has resulted in a sharp increase in the number of species with genome assemblies. However, these assemblies are highly fragmented, with many gaps, ambiguities, and errors, impeding downstream applications. We demonstrate current state of the art for de novo assembly using the domestic goat (Capra hircus) based on long reads for contig formation, short reads for consensus validation, and… CONTINUE READING
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