Single-molecule resolution fluorescent in situ hybridization (smFISH) in the yeast S. cerevisiae.

@article{Rahman2013SinglemoleculeRF,
  title={Single-molecule resolution fluorescent in situ hybridization (smFISH) in the yeast S. cerevisiae.},
  author={Samir Rahman and Daniel Zenklusen},
  journal={Methods in molecular biology},
  year={2013},
  volume={1042},
  pages={33-46}
}
Regulating gene expression is a major task for all cellular systems. RNA production and degradation plays a critical role in this process and accurately measuring cellular mRNA levels is essential to understanding gene expression regulation. Classical biochemical assays that study gene expression rely on extracting RNAs from large populations of cells, taking them out of their native context and thereby losing spatial information as well as cell-to-cell variability. In this chapter, we describe… CONTINUE READING
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