Single-molecule real-time transcript sequencing facilitates common wheat genome annotation and grain transcriptome research

@inproceedings{Dong2015SinglemoleculeRT,
  title={Single-molecule real-time transcript sequencing facilitates common wheat genome annotation and grain transcriptome research},
  author={Lingli Dong and Hongfang Liu and Juncheng Zhang and Shuangjuan Yang and Guanyi Kong and Jeffrey S. C. Chu and Nansheng Chen and Dao Wen Wang},
  booktitle={BMC Genomics},
  year={2015}
}
The large and complex hexaploid genome has greatly hindered genomics studies of common wheat (Triticum aestivum, AABBDD). Here, we investigated transcripts in common wheat developing caryopses using the emerging single-molecule real-time (SMRT) sequencing technology PacBio RSII, and assessed the resultant data for improving common wheat genome annotation and grain transcriptome research. We obtained 197,709 full-length non-chimeric (FLNC) reads, 74.6 % of which were estimated to carry complete… CONTINUE READING
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