Single-molecule nonequilibrium periodic Mg2+-concentration jump experiments reveal details of the early folding pathways of a large RNA.

@article{Qu2008SinglemoleculeNP,
  title={Single-molecule nonequilibrium periodic Mg2+-concentration jump experiments reveal details of the early folding pathways of a large RNA.},
  author={Xiaohui Qu and Glenna J Smith and Kang Taek Lee and Tobin R. Sosnick and Tao Pan and Norbert F. Scherer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2008},
  volume={105 18},
  pages={6602-7}
}
The evolution of RNA conformation with Mg(2+) concentration ([Mg(2+)]) is typically determined from equilibrium titration measurements or nonequilibrium single [Mg(2+)]-jump measurements. We study the folding of single RNA molecules in response to a series of periodic [Mg(2+)] jumps. The 260-residue catalytic domain of RNase P RNA from Bacillus stearothermophilus is immobilized in a microfluidic flow chamber, and the RNA conformational changes are probed by fluorescence resonance energy… CONTINUE READING
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